g418 (MedChemExpress)
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MedChemExpress
g418
G418, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/g+418+disulfate/G-418+disulfate/pm42486991-480-11-12
Average 95 stars, based on 69 article reviews
G418, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/g+418+disulfate/G-418+disulfate/pm42486991-480-11-12
Average 95 stars, based on 69 article reviews
g418 - by Bioz Stars,
2026-09
95/100 stars
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Transfection:Article Title: MBD2 regulates the progression and chemoresistance of cholangiocarcinoma through interaction with WDR5 Article Snippet: The culture supernatant was collected after 72 h and filtered through a 0.45 μm membrane (BS-PES-45; Biosharp). .. TFK1 and RBE cells were transfected with 1 mL of lentivirus supernatant and cultured in 1 mL of complete RPMI 1640 medium and 2 μL of polybrene (40804ES76; Yeasen) for 24 h. After 72 h, puromycin (CL13900; Selleck, 1 μg/mL) or Article Title: Tongguanteng injection exerts anti-osteosarcoma effects through the ER stress-associated IRE1/CHOP pathway. Article Snippet: After 6 h of transfection, the media was replaced with fresh DMEM complete media (HyClone, USA) and cultured for 48 h. Then the proteins were extracted and the transfection efficiency was calculated via a western blot experiment. .. However, cells transfected with IRE1-OE plasmid were screened with 200 μg/mL Article Title: RNA-binding protein HnRNPU regulates proliferation and ferroptosis in colon adenocarcinoma by stabilizing the mRNA of system xc − Article Snippet: Human Lenti-sh-HnRNPU-mNeonGreen, Lenti-oeSLC3A2-mScarlet, Lenti-oeSLC7A11-RFP and control groups were transfected (Beijing SyngenTech). .. Puromycin dihydrochloride (Beyotime, cat. no. ST551), Article Title: NUSAP1 Recruits DAXX to Suppress HIF‐Driven Triple‐Negative Breast Cancer Progression Article Snippet: [ ] Briefly, HEK293T cells were co‐transfected with individual expression plasmids and packaging vectors pMD2.G and psPAX2 using Polyjet transfection reagent (SignaGen, SL100688). .. Forty‐8 h after transfection, the virus particles were collected, filtered using a 0.45 μm syringe filter, and then transduced into breast cancer cells for co‐culture for 48 h. Subsequently, selection was performed using puromycin dihydrochloride (1.0 μg mL −1 , Abcam, ab141453) or Article Title: Tongguanteng injection exerts anti-osteosarcoma effects through the ER stress-associated IRE1/CHOP pathway Article Snippet: After 6 h of transfection, the media was replaced with fresh DMEM complete media (HyClone, USA) and cultured for 48 h. Then the proteins were extracted and the transfection efficiency was calculated via a western blot experiment. .. However, cells transfected with IRE1-OE plasmid were screened with 200 μg/mL Article Title: RNA-binding protein HnRNPU regulates proliferation and ferroptosis in colon adenocarcinoma by stabilizing the mRNA of system xc . Article Snippet: Human Lenti-sh-HnRNPU-mNeonGreen, Lenti-oeSLC3A2-mScarlet, Lenti-oeSLC7A11-RFP and control groups were transfected (Beijing SyngenTech). .. Puromycin dihydrochloride (Beyotime, cat. no. ST551), Article Title: NUSAP1 Recruits DAXX to Suppress HIF-Driven Triple-Negative Breast Cancer Progression. Article Snippet: [55] Briefly, HEK293T cells were co-transfected with individual expression plasmids and packaging vectors pMD2.G and psPAX2 using Polyjet transfection reagent (SignaGen, SL100688). .. Forty-8 h after transfection, the virus particles were collected, filtered using a 0.45 μm syringe filter, and then transduced into breast cancer cells for co-culture for 48 h. Subsequently, selection was performed using puromycin dihydrochloride (1.0 μgmL−1, Abcam, ab141453) or Cell Culture:Article Title: MBD2 regulates the progression and chemoresistance of cholangiocarcinoma through interaction with WDR5 Article Snippet: The culture supernatant was collected after 72 h and filtered through a 0.45 μm membrane (BS-PES-45; Biosharp). .. TFK1 and RBE cells were transfected with 1 mL of lentivirus supernatant and cultured in 1 mL of complete RPMI 1640 medium and 2 μL of polybrene (40804ES76; Yeasen) for 24 h. After 72 h, puromycin (CL13900; Selleck, 1 μg/mL) or Plasmid Preparation:Article Title: Tongguanteng injection exerts anti-osteosarcoma effects through the ER stress-associated IRE1/CHOP pathway. Article Snippet: After 6 h of transfection, the media was replaced with fresh DMEM complete media (HyClone, USA) and cultured for 48 h. Then the proteins were extracted and the transfection efficiency was calculated via a western blot experiment. .. However, cells transfected with IRE1-OE plasmid were screened with 200 μg/mL Article Title: Tongguanteng injection exerts anti-osteosarcoma effects through the ER stress-associated IRE1/CHOP pathway Article Snippet: After 6 h of transfection, the media was replaced with fresh DMEM complete media (HyClone, USA) and cultured for 48 h. Then the proteins were extracted and the transfection efficiency was calculated via a western blot experiment. .. However, cells transfected with IRE1-OE plasmid were screened with 200 μg/mL Stable Transfection:Article Title: Tongguanteng injection exerts anti-osteosarcoma effects through the ER stress-associated IRE1/CHOP pathway. Article Snippet: After 6 h of transfection, the media was replaced with fresh DMEM complete media (HyClone, USA) and cultured for 48 h. Then the proteins were extracted and the transfection efficiency was calculated via a western blot experiment. .. However, cells transfected with IRE1-OE plasmid were screened with 200 μg/mL Article Title: Tongguanteng injection exerts anti-osteosarcoma effects through the ER stress-associated IRE1/CHOP pathway Article Snippet: After 6 h of transfection, the media was replaced with fresh DMEM complete media (HyClone, USA) and cultured for 48 h. Then the proteins were extracted and the transfection efficiency was calculated via a western blot experiment. .. However, cells transfected with IRE1-OE plasmid were screened with 200 μg/mL Western Blot:Article Title: Tongguanteng injection exerts anti-osteosarcoma effects through the ER stress-associated IRE1/CHOP pathway. Article Snippet: After 6 h of transfection, the media was replaced with fresh DMEM complete media (HyClone, USA) and cultured for 48 h. Then the proteins were extracted and the transfection efficiency was calculated via a western blot experiment. .. However, cells transfected with IRE1-OE plasmid were screened with 200 μg/mL Article Title: Tongguanteng injection exerts anti-osteosarcoma effects through the ER stress-associated IRE1/CHOP pathway Article Snippet: After 6 h of transfection, the media was replaced with fresh DMEM complete media (HyClone, USA) and cultured for 48 h. Then the proteins were extracted and the transfection efficiency was calculated via a western blot experiment. .. However, cells transfected with IRE1-OE plasmid were screened with 200 μg/mL Infection:Article Title: Recurrence in the chemotherapy regimen of bladder carcinoma originates from quiescent epidermoid-like cells. Article Snippet: .. Briefly, for both MGH-U3-LT and RT4LT, the cells were first infected with luciferase-neomycin lentivirus; two days following infection, neomycin (+) cells were selected by treatment with 1000 μg/mL Luciferase:Article Title: Recurrence in the chemotherapy regimen of bladder carcinoma originates from quiescent epidermoid-like cells. Article Snippet: .. Briefly, for both MGH-U3-LT and RT4LT, the cells were first infected with luciferase-neomycin lentivirus; two days following infection, neomycin (+) cells were selected by treatment with 1000 μg/mL Clone Assay:Article Title: Recurrence in the chemotherapy regimen of bladder carcinoma originates from quiescent epidermoid-like cells. Article Snippet: .. Briefly, for both MGH-U3-LT and RT4LT, the cells were first infected with luciferase-neomycin lentivirus; two days following infection, neomycin (+) cells were selected by treatment with 1000 μg/mL Virus:Article Title: NUSAP1 Recruits DAXX to Suppress HIF‐Driven Triple‐Negative Breast Cancer Progression Article Snippet: [ ] Briefly, HEK293T cells were co‐transfected with individual expression plasmids and packaging vectors pMD2.G and psPAX2 using Polyjet transfection reagent (SignaGen, SL100688). .. Forty‐8 h after transfection, the virus particles were collected, filtered using a 0.45 μm syringe filter, and then transduced into breast cancer cells for co‐culture for 48 h. Subsequently, selection was performed using puromycin dihydrochloride (1.0 μg mL −1 , Abcam, ab141453) or Article Title: NUSAP1 Recruits DAXX to Suppress HIF-Driven Triple-Negative Breast Cancer Progression. Article Snippet: [55] Briefly, HEK293T cells were co-transfected with individual expression plasmids and packaging vectors pMD2.G and psPAX2 using Polyjet transfection reagent (SignaGen, SL100688). .. Forty-8 h after transfection, the virus particles were collected, filtered using a 0.45 μm syringe filter, and then transduced into breast cancer cells for co-culture for 48 h. Subsequently, selection was performed using puromycin dihydrochloride (1.0 μgmL−1, Abcam, ab141453) or Selection:Article Title: NUSAP1 Recruits DAXX to Suppress HIF‐Driven Triple‐Negative Breast Cancer Progression Article Snippet: [ ] Briefly, HEK293T cells were co‐transfected with individual expression plasmids and packaging vectors pMD2.G and psPAX2 using Polyjet transfection reagent (SignaGen, SL100688). .. Forty‐8 h after transfection, the virus particles were collected, filtered using a 0.45 μm syringe filter, and then transduced into breast cancer cells for co‐culture for 48 h. Subsequently, selection was performed using puromycin dihydrochloride (1.0 μg mL −1 , Abcam, ab141453) or Article Title: NUSAP1 Recruits DAXX to Suppress HIF-Driven Triple-Negative Breast Cancer Progression. Article Snippet: [55] Briefly, HEK293T cells were co-transfected with individual expression plasmids and packaging vectors pMD2.G and psPAX2 using Polyjet transfection reagent (SignaGen, SL100688). .. Forty-8 h after transfection, the virus particles were collected, filtered using a 0.45 μm syringe filter, and then transduced into breast cancer cells for co-culture for 48 h. Subsequently, selection was performed using puromycin dihydrochloride (1.0 μgmL−1, Abcam, ab141453) or |